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Chemical characterization

In-depth chemical characterisation of biomass

Chemical characterisation of biomass and bio-based materials requires knowledge about the material itself and which technique to use for analysing a given compound. RISE PFI has through our various research projects built a broad experience about characterisation of a wide range of biomasses. Our selection of state-of-the art instruments combined with a series of well-established characterisation techniques enables us to characterise the composition of well-known and emerging biomasses.


chemical characterisation of biomass
Selected equipment
  • Gas Chromatography (GC)
  • High Pressure Liquid Chromatography (HPLC).
  • Pyrolysis GC-MS (with µ-Tandem reactor)
  • Size Exclusion Chromatography, semi preparative (SEC)
  • UV-Visible Spectroscopy (UV-VIS)
  • Dr. Lange photometer
  • Oxygen permeation measurement system (OX-TRAN)
  • Particle charge detector (Mütek PCD 02)
  • Simultaneous thermal analyzer (STA – TGA/DSC)
  • Fourier-transform infrared spectroscopy (FTIR)

Selected methods

Extractive content
Water – Gravimetric determination.PFI-method-05-P-15
Water – Quantitative analysis, group separation.PFI-method-05-P-13
Water – Quantitative analysis , single components.PFI-method-05-P-16
Pulp & paper – Gravimetric determination – AcetoneSCAN-CM 49:03
Pulp & paper – Gravimetric determination – Lipophilic matterSCAN-CM 67:03
Pulp & paper – Quantitative analysis, group separationPFI-method-05-P-13
Pulp & paper – Quantitative analysis , single componentsPFI-method-05-P-22
Water – Total carbohydrates, the phenol-sulphuric acid  method.PFI-method-05-P-11
Water – Hemicelluloses  and uronic acid, methanolysis.PFI-method-05-P-12
Water – Quantitative composition, cellulose and hemicellulose.PFI-method-05-P-17
Water – Reducing sugars, Nelsons method.PFI-method-05-P-18
Pulp & paper – Hemicelluloses  and uronic acid, methanolysisPFI-method-05-P-12
Pulp & paper – Quantitative composition, cellulose and hemicellulose.PFI-method-05-P-23
Water – Compounds in the water phase after MTBE-extraction.PFI-method-05-P-19
Pulp & paper – Acid insoluble (Klason) and acid soluble ligninPFI-method-05-P-24
Other analyses
Water – Particle sizePFI-method-05-P-21
Water – ColourNS 4787
Water – Suspended matter and residue on ignitionNS 4733
Water – Dry content and residue on ignitionNS 4764
Water – pHNS 4720
Water – ConductivityNS 4721
Pulp & paper – Total charge.PFI-method-05-P-25
Pulp & paper – Total charge – Total acidic group content.SCAN CM 65:02
Pulp & paper – Surface chargePFI-method-05-P-26
Sulfonic acidPFI-method-05-P-27
Volatile compounds: (based on “purge & trap”-GC/MS)PFI-method
Dry residue, HR73 Moisture Analyzer (balance)PFI-method-05-P-A-02
Ash contentISO 1762
Deposits:  Quantitative analysis – py-GC/MS and FT-IR
MFC-films: OTR, oxygen transmission ratePFI-method-05-P-28

Chemical characterization

RISE PFI is part of RISE - Research Institutes of Sweden

RISE PFI is and internationally acknowledged research institute within processes and products based on lignocellulose. RISE PFI´s focus areas are Fibre technology and fibre-based products, Biorefining and Bioenergy and Biopolymers and Biocomposites.



  • Høgskoleringen 6b
  • NO - 7491 Trondheim
  • firmapost@rise-pfi.no
  • +47 73 60 50 65